perm buffer (10X Genomics)
86
Structured Review
10X Genomics
perm buffer
Perm Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/buffer+perm/pm42106323-210-2-4
Average 86 stars, based on 1 article reviews
Perm Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/buffer+perm/pm42106323-210-2-4
Average 86 stars, based on 1 article reviews
perm buffer - by Bioz Stars,
2026-10
86/100 stars
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Staining:Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development. Article Snippet: Cells from 3 patients (patient IDs 119, 158 and 236) were thawed, counted and 7.5 × 105 cells were cultured as described above, 1.5 mL per well in media (complete DMEM) alone in 24-well plates (Costar 3526) and incubated at 37 ◦C in 5% CO2 for 24 h. The media was aspirated from the wells and immediately replaced with media containing pioglitazone 10 μM, LPS 50 ng/mL or DMSO 1 μL/mL and incubated at 37 ◦C in 5% CO2 for 72 h. Cells were then harvested using trypsin-EDTA as described, counted, and each divided 4 ways for an RNA sample resuspended in 300 μL of RLT lysis buffer (Qiagen RNeasy micro kit) and stored at −20 ◦C for later RNA extraction, or re-cultured and incubated for a further 72 h with pioglitazone 10 μM, LPS 50 ng/mL or DMSO 1 μL/mL in 150 μL per well in 96-well flat-bottomed plate (Costar 3596). .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Concentration Assay:Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development. Article Snippet: Cells from 3 patients (patient IDs 119, 158 and 236) were thawed, counted and 7.5 × 105 cells were cultured as described above, 1.5 mL per well in media (complete DMEM) alone in 24-well plates (Costar 3526) and incubated at 37 ◦C in 5% CO2 for 24 h. The media was aspirated from the wells and immediately replaced with media containing pioglitazone 10 μM, LPS 50 ng/mL or DMSO 1 μL/mL and incubated at 37 ◦C in 5% CO2 for 72 h. Cells were then harvested using trypsin-EDTA as described, counted, and each divided 4 ways for an RNA sample resuspended in 300 μL of RLT lysis buffer (Qiagen RNeasy micro kit) and stored at −20 ◦C for later RNA extraction, or re-cultured and incubated for a further 72 h with pioglitazone 10 μM, LPS 50 ng/mL or DMSO 1 μL/mL in 150 μL per well in 96-well flat-bottomed plate (Costar 3596). .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Incubation:Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development. Article Snippet: Cells from 3 patients (patient IDs 119, 158 and 236) were thawed, counted and 7.5 × 105 cells were cultured as described above, 1.5 mL per well in media (complete DMEM) alone in 24-well plates (Costar 3526) and incubated at 37 ◦C in 5% CO2 for 24 h. The media was aspirated from the wells and immediately replaced with media containing pioglitazone 10 μM, LPS 50 ng/mL or DMSO 1 μL/mL and incubated at 37 ◦C in 5% CO2 for 72 h. Cells were then harvested using trypsin-EDTA as described, counted, and each divided 4 ways for an RNA sample resuspended in 300 μL of RLT lysis buffer (Qiagen RNeasy micro kit) and stored at −20 ◦C for later RNA extraction, or re-cultured and incubated for a further 72 h with pioglitazone 10 μM, LPS 50 ng/mL or DMSO 1 μL/mL in 150 μL per well in 96-well flat-bottomed plate (Costar 3596). .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Hybridization:Article Title: Catecholaminergic neurons boost fibroblast osteogenic activity in keloid. Article Snippet: .. Fix & Sequencing:Article Title: Disruption of immune responses by type 1 diabetes exacerbates SARS-CoV-2 mediated lung injury Article Snippet: .. Samples dedicated to scRNA sequencing were fixed with Fix & Flow Cytometry:Article Title: Disruption of immune responses by type 1 diabetes exacerbates SARS-CoV-2 mediated lung injury Article Snippet: .. Samples dedicated to scRNA sequencing were fixed with Fix & |